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anti p mlc2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti p mlc2
    Anti P Mlc2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1390 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+mlc2+ser19/Phospho-Myosin+Light+Chain+2+(Ser19)+Antibody/pm41832874-83-28-30
    Average 96 stars, based on 1390 article reviews
    anti p mlc2 - by Bioz Stars, 2026-09
    96/100 stars

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    other:

    Article Title: The intellectual disability protein Oligophrenin-1 controls astrocyte morphology and migration.
    Article Snippet: We used the antibodies against: MLC2 (Cell Signaling Technology (CST), (Leiden, The Netherlands), Rabbit, reference 8505S) at 1:500, P-MLC2 (Ser19) (CST, Rabbit, reference 3671S) at 1:500, Nestin (Merck-Millipore (Molsheim, France), Mouse, reference MAB353) at 1:200, GFAP (Sigma-Aldrich (Saint-Quentin Fallavier, France), Mouse, reference G3893) at 1:500, Vinculin (Invitrogen (Carlsbad, CA, USA), Rabbit, reference 42H89L44) at 1:200, Phospho-histone H3 (Ser10) (Merck-Millipore (Molsheim, France), Rabbit, reference 06-570) at 1:200, cleaved Caspase3 (CST, Rabbit, reference 9661) at 1:200, GFP (green fluorescent protein, Abcam, (Cambridge, UK) Chicken, reference ab13970) at 1:500, GLT-1 (glutamate-transporter-1, Frontier Institute (Hokkaido, Japan), Rabbit, reference Af670) at 1:500, GAPDH (glyceraldehyde-3-phosphate dehydrogenase, Sigma-Aldrich (SaintQuentin Fallavier, France), reference G9295 at 1:10000), OPHN1 (crude rabbit serum; Fauchereau et al., 2003) at 1:500, and glutamine synthase (GS) (BD transduction laboratories, (NULL, USA) Mouse, reference 45020) at 1:500, S100β (Sigma-Aldrich, (Saint-Quentin Fallavier, France), Rabbit, reference S2644) at 1:500.

    Article Title: Effects of stanniocalcin-1 overexpressing hepatocellular carcinoma cells on macrophage migration
    Article Snippet: p-MLC2 (Ser19) , Cell Signaling , 3671T , Rabbit.

    Article Title: Deficiency of CPEB2-Confined Choline Acetyltransferase Expression in the Dorsal Motor Nucleus of Vagus Causes Hyperactivated Parasympathetic Signaling-Associated Bronchoconstriction
    Article Snippet: Antibodies used were ChAT (Millipore, catalog #AB144P; RRID:AB_2079751), neurofilament heavy chain (NF-H; Millipore, catalog #AB5539; RRID:AB_177520), tyrosine hydroxylase (TH; Millipore, catalog #AB152; RRID:AB_390204), α-smooth muscle actin (αSMA; Abcam, catalog #ab7817; RRID:AB_262054), neurokinin 1 receptor 1 (NK1R; Sigma-Aldrich, catalog #S8305; RRID:AB_261562), β-actin (Sigma-Aldrich, catalog #A5441; RRID:AB_476744), α-tubulin (Sigma-Aldrich, catalog #T5168; RRID:AB_477579), p-Mlc2/Ser19 (Cell Signaling Technology, catalog #3671S; RRID:AB_330248), and β3-tubulin (Cell Signaling Technology, catalog #5666P; RRID:AB_10691594).

    Incubation:

    Article Title: The Hippo pathway links adipocyte plasticity to adipose tissue fibrosis
    Article Snippet: .. Membranes were blotted with primary antibodies against HSP90 (Santa Cruz, sc-13119, 1:10,000), α-Tubulin (Sigma, T6199, 1:10,000), Lamin B1 (Proteintech, 66095-1-Ig, 1:10,000), LATS1 (Cell Signaling, 3477, 1:1000), LATS2 (Bethyl Laboratories, A300-479A, 1:1000), YAP (Cell Signaling, 4912 or 14074, 1:1000), TAZ (Cell Signaling, 4883 or 83669, 1:1000), p-YAP (Ser 112) (Cell Signaling, 4911), p-TAZ (Ser 89) (Cell Signaling, 59971, 1:1000), HSL (Cell Signaling, 4107, 1:1000), Perilipin 1 (Vala Sciences, 4854, 1:10,000), SMAD2/3 (Cell Signaling, 8685, 1:1000), p-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) (Cell Signaling, 8828, 1:1000), αSMA (Cell Signaling, 19245, 1:5000), FLAG (Abmart, M20008, 1:5000), Caspase 3 (Cell Signaling, 9662,1:1000), MST1 (Cell Signaling, 3682, 1:1000), MST2 (Cell Signaling, 3952, 1:1000), p-SAPK/JNK (Thr183/Tyr185) (Cell Signaling, 4668, 1:1000), SAPK/JNK (Cell Signaling, 9252, 1:1000), p-AKT (Ser473) (Cell Signaling, 4060, 1:1000), AKT (Cell Signaling, 9272, 1:1000), p-p38 MAPK (Thr180/Tyr182) (Cell Signaling, 4511, 1:1000), p38 MAPK (Cell Signaling, 8690, 1:1000), p-MLC2 (Ser19) (Cell Signaling, 3671, 1:1000), MLC2 (Proteintech, 10906-1-AP, 1:1000), ERK1/ERK2 (ABclonal, A10613, 1:1000), p-ERK1(T202/Y204)/ERK2(T185/Y187) (ABclonal, AP0472, 1:1000), GFP (Thermo Scientific, A-11120, 1:1000), Myc (Cell Signaling, 2276, 1:2000) and Col1a1 (Cell Signaling, 72026, 1:1000) followed by incubation with HRP-conjugated anti-rabbit secondary antibody (Thermo Scientific, 31460, 1:10,000) or anti-mouse secondary antibody (Thermo Scientific, 32430, 1:10,000). .. Nuclear and cytoplasmic fractionation were performed using the Nuclear and Cytoplasmic Protein Extraction Kit (Beyotime, P0028), according to the manufacturer’s manual.



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    anti p mlc2  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc anti p mlc2
    Anti P Mlc2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+mlc2+ser19/Phospho-Myosin+Light+Chain+2+(Ser19)+Antibody/pm41832874-83-28-30
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    rabbit anti p mlc2  (Cell Signaling Technology Inc)
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    p mlc2  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc p mlc2
    Inhibition of MLCK signaling mimicked the effects of DHA and EPA on the proportion of zipper-like junctions in PA-treated LECs. (A-D) Effects of ML-7 (50 μM) treatment on the proportion of zipper-like junction proportion (A, B) and the expression of MLCK, <t>p-MLC2</t> (C, D) in PA-treated (100 μM) LECs. White dashed line: VE-cadherin-negative junctions; white arrow: button-like junctions; yellow arrow: zipper-like junctions. Two-tailed unpaired Student’s t -test was performed to compare PA versus PA + DHA/EPA/ML-7-treated groups at the same time points. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
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    appliacation (dilutions) p-mlc2 (s19) cst 3675 wb  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc appliacation (dilutions) p-mlc2 (s19) cst 3675 wb
    Inhibition of MLCK signaling mimicked the effects of DHA and EPA on the proportion of zipper-like junctions in PA-treated LECs. (A-D) Effects of ML-7 (50 μM) treatment on the proportion of zipper-like junction proportion (A, B) and the expression of MLCK, <t>p-MLC2</t> (C, D) in PA-treated (100 μM) LECs. White dashed line: VE-cadherin-negative junctions; white arrow: button-like junctions; yellow arrow: zipper-like junctions. Two-tailed unpaired Student’s t -test was performed to compare PA versus PA + DHA/EPA/ML-7-treated groups at the same time points. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
    Appliacation (Dilutions) P Mlc2 (S19) Cst 3675 Wb, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Inhibition of MLCK signaling mimicked the effects of DHA and EPA on the proportion of zipper-like junctions in PA-treated LECs. (A-D) Effects of ML-7 (50 μM) treatment on the proportion of zipper-like junction proportion (A, B) and the expression of MLCK, p-MLC2 (C, D) in PA-treated (100 μM) LECs. White dashed line: VE-cadherin-negative junctions; white arrow: button-like junctions; yellow arrow: zipper-like junctions. Two-tailed unpaired Student’s t -test was performed to compare PA versus PA + DHA/EPA/ML-7-treated groups at the same time points. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Journal: Journal of Advanced Research

    Article Title: Fish oil reduces intestinal fat absorption by promoting lacteal junction zippering via GPR120-VEGFR3-MLCK pathway

    doi: 10.1016/j.jare.2025.05.026

    Figure Lengend Snippet: Inhibition of MLCK signaling mimicked the effects of DHA and EPA on the proportion of zipper-like junctions in PA-treated LECs. (A-D) Effects of ML-7 (50 μM) treatment on the proportion of zipper-like junction proportion (A, B) and the expression of MLCK, p-MLC2 (C, D) in PA-treated (100 μM) LECs. White dashed line: VE-cadherin-negative junctions; white arrow: button-like junctions; yellow arrow: zipper-like junctions. Two-tailed unpaired Student’s t -test was performed to compare PA versus PA + DHA/EPA/ML-7-treated groups at the same time points. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: Primary antibodies include CD36 (proteintech,18836–1-AP), VEGFC (proteintech,67116–1-Ig), VEGFR3 (R&D, AF743), ApoB48 (ThermoFisher, MA5-35458), MTTP (ThermoFisher, PA5-102474,), MLCK (ThermoFisher Scientific, PA5100908), GPR120 (Santa Cruz, sc-9901), p-MLC2 (CST, 95777S, Thr18/Ser19), GAPDH (Bioworld, AP0063), β-actin (Bioworld, AP0060) and β-Tubulin (Bioworld, AP0064).

    Techniques: Inhibition, Expressing, Two Tailed Test